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Regulation of chloroplast proteome dynamics, biogenesis and physiology

Published on 1 September 2016

Team leader

Norbert Rolland
PhD, CNRS Research Director
Team leader
Director of the Cell & Plant Physiology Laboratory

Phone: 04 38 78 49 86
Fax: 04 38 78 50 91


Laboratoire Physiologie Cellulaire & Végétale
17 avenue des Martyrs
38 054 Grenoble cedex 9

Members of the team

Imen Bouchnak: INRA/Labex GRAL PhD thesis
Marcel Kuntz: CNRS Research Director
Lucas Moyet: INRA Assistant Engineer
Norbert Rolland: CNRS Research Director
Daniel Salvi: CNRS Engineer

Former members of the team


The chloroplast the place of production of a large number of biomolecules (fatty acids, amino acids, sugars, vitamins, terpenes, alkaloids, phenols etc.) that have a great interest for bioenergy, for food and for human health. As the main source of energy for the plant cell, this photosynthetic organelle is essential for the synthesis of the whole plant biomass, and is the main "CO
2 pump", thus also acting on the environment and climate.

Research activities

One of the most fascinating aspects (and least studied) of photosynthetic organisms is their ability to manage rapid changes in adverse environmental conditions, and to reorient their metabolism in response to what is usually denominated "stress" (light, nutrients, cold, etc...). The fate of the plant, under the different conditions it will encounter during its life cycle, relies on the speed and the effectiveness of these responses. These types of response involve short-term adaptation changes, longer-term changes in gene expression and thus protein composition. Our approaches aim to decipher the regulatory networks involved and to better understand the ability of plants to respond to changing conditions of the environment.

Methods used in the team are, large-scale proteomics (in collaboration with colleagues from the BGE laboratory), reverse genetics in plants, confocal microscopy, HPLC detection of pigments and vitamins, functional characterization of chloroplast ion transporters, biochemistry, enzymology, structural biology. Analytical modeling approaches are also used to integrate data from cell biology to biochemistry. Our favorite model organisms are higher plants (Arabidopsis, tomato, spinach…).

Research activities: Selected recent highlights

Publications of the team

Selected publications

Sautron E, Giustini C, Dang T, Moyet L, Salvi D, Crouzy S, Rolland N, Catty P and Seigneurin-Berny D
Identification of two conserved residues involved in copper release from chloroplast PIB-1-ATPases.
Journal of Biological Chemistry, 2016, 291(38): 20136–20148

Tomizioli M, Lazar C, Brugiere S, Burger T, Salvi D, Gatto L, Moyet L, Breckels LM, Hesse AM, Lilley KS, Seigneurin-Berny D, Finazzi G, Rolland N and Ferro M
Deciphering thylakoid sub-compartments using a mass spectrometry-based approach.
Molecular and Cellular Proteomics, 2014, 13(8): 2147-2167

Rolland N, Curien G, Finazzi G, Kuntz M, Maréchal E, Matringe M, Ravanel S and Seigneurin-Berny D
The biosynthetic capacities of the plastids and integration between cytoplasmic and chloroplast processes.
Annual Review of Genetics, 2012, 46: 233-264

Tardif M, Atteia A, Specht M, Cogne G, Rolland N, Brugiere S, Hippler M, Ferro M, Bruley C, Peltier G, Vallon O and Cournac L
PredAlgo, a new subcellular localization prediction tool dedicated to green algae.
Molecular Biology and Evolution, 2012, 29(12): 3625-3639

Catty P, Boutigny S, Miras R, Joyard J, Rolland N and Seigneurin-Berny D
Biochemical characterization of AtHMA6/PAA1, a chloroplast envelope Cu(I)-ATPase.
Journal of Biological Chemistry, 2011, 286(42): 36188–36197

Ferro M, Brugiere S, Salvi D, Seigneurin-Berny D, Court M, Moyet L, Ramus C, Miras S, Mellal M, Le Gall S, Kieffer-Jaquinod S, Bruley C, Garin J, Joyard J, Masselon C and Rolland N
AT_CHLORO: A comprehensive chloroplast proteome database with subplastidiallocalization and curated information on envelope proteins.
Molecular and Cellular Proteomics, 2010, 9(1): 1063-1084

Joyard J, Ferro M, Masselon C, Seigneurin-Berny D, Salvi D, Garin J and Rolland N
Chloroplast proteomics highlights the subcellular compartmentation of lipid metabolism.
Progress in Lipid Research, 2010, 49(2): 128-158

Atteia A, Adrait A, Brugiere S, Tardif M, van Lis R, Deusch O, Dagan T, Kuhn L, Gontero B, Martin W, Garin J, Joyard J and Rolland N
A proteomic survey of Chlamydomonas reinhardtii mitochondria sheds new light on the metabolic plasticity of the organelle and on the nature of the α-proteobacterial mitochondrial ancestor.
Molecular Biology and Evolution, 2009, 26(7): 1533-1548

Miras S, Salvi D, Piette L, Seigneurin-Berny D, Grunwald D, Reinbothe C, Joyard J, Reinbothe S and Rolland N
TOC159- and TOC75-independent import of a transit sequence-less precursor into the inner envelope of chloroplasts.
Journal of Biological Chemistry, 2007, 282(40): 29482-29492

Seigneurin-Berny D, Gravot A, Auroy P, Mazard C, Kraut A, Finazzi G, Grunwald D, Rappaport F, Vavasseur A, Joyard J, Richaud P and Rolland N
HMA1, a new Cu-ATPase of the chloroplast envelope, is essential for growth under adverse light conditions.
Journal of Biological Chemistry, 2006, 281(5): 2882-2892

Villarejo A, Burén S, Larsson S, Déjardin A, Monné M, Rudhe C, Karlsson J, Jansson S, Lerouge P, Rolland N, von Heijne G, Grebe M, Bako L and Samuelsson G
Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast.
Nature Cell Biology, 2005, 7(12): 1224-1231

Ferro M, Salvi D, Brugiere S, Miras S, Kowalski S, Louwagie M, Garin J, Joyard J and Rolland N
Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana.
Molecular and Cellular Proteomics, 2003, 2(5): 325-345

Ferro M, Salvi D, Rivière-Rolland H, Vermat T, Seigneurin-Berny D, Grunwald D, Garin J, Joyard J and Rolland N
Integral membrane proteins of the chloroplast envelope: Identification and subcellular localization of new transporters.
Proceedings of the National Academy of Sciences USA, 2002, 99: 11487-11492

Miras S, Salvi D, Ferro M, Grunwald D, Garin J, Joyard J and Rolland N
Non canonical transit peptide for import into the chloroplast.
Journal of Biological Chemistry, 2002, 277: 47770-47778


Chloroplast, Metabolism, Chloroplast envelope, Transports, ATPases, Copper, Chloroplast biogenesis, Plant, Physiology, Biochemistry, Proteomics, Systems Biology.

Present collaborative projects are supported by:

• Grant INRA & Labex GRAL. Project "Chloroplast biogenesis". Project coordinator Norbert Rolland. 2015-2018.
• Grant INRA. Project "HMA1". Team coordinators Daphné Berny/Norbert Rolland. 2014-2015.
• Grant PhD Labex GRAL & CEA. Project "ATPases". Project coordinator Daphné Berny. 2012-2015.
• Grant Engineer Labex GRAL. Project "Chloroplast". Project Coordinator Norbert Rolland. 2012-2015.

Former funding

• Grant ANR Genomics and Plant Biotechnologies. Project "Chloro-Types". Project Coordinator Norbert Rolland. 2011-2014.
• Grant ANR Blanc 2010. Project "Chloro-Pro". Coordinator Norbert Rolland. 2011-2014.

• Grant PhD CEA IRTELIS. Project coordinators Gilles Curien/Norbert Rolland. 2010-2013.
• Grant INRA. Project "Fib". Project coordinators Michel Matringe/Marcel Kuntz. 2011.
• Grant ANR Génoplante. Project "Glyco-Chloroplast". Team coordinator Norbert Rolland. 2007-2010.
• Grant CNRS Post-doc. Project “Membrane proteins”. Project coordinator Norbert Rolland. 2008-2009.
• Grant CEA. Project "CEA-PM: Screening of Alternative Expression systems for the Production of Membrane Proteins". Project coordinator Norbert Rolland. 2007-2009.
• Grant PhD CEA. Project “Atpases”. Project coordinators Daphné Berny/Norbert Rolland. 2006-2009.
• Grant CNRS Post-doc. Project “Chlamydomonas proteomics”. Project coordinator Norbert Rolland. 2005-2006.
• Grant Rhône-Alpes Region/CEA. Project "Development of an in vitro expression technology for the production of membrane proteins". Project coordinator Norbert Rolland. 2003-2005.
• Grant Génoplante 2 - New Tools (phase 2). Project "Characterization of Arabidopsis thaliana gene products: Hydrophobic proteome of membranes". Project coordinator Norbert Rolland. 2002 -2005.
• Grant PhD CEA. Project “Alternative chloroplast targeting pathways”. Project coordinator Norbert Rolland. 2001-2004.
• Grant Génoplante 2 - New tools. Project "Chloroplast microarrays". Project coordinator Norbert Rolland. 2001-2003.
• Grant CEA National Program of Nuclear Toxicology. Project "Impact of radionuclides on the metabolism of higher plants". Project coordinator Norbert Rolland. 2001-2003.
• Grant Génoplante I - Functional Analyses (AF 1999-035). Project "Functional genomics of membrane transport systems in Arabidopsis thaliana". Team coordinator Norbert Rolland. 2000-2001.
• Grant Génoplante I - New tools (NO 1999-3663). Project "Characterization of Arabidopsis thaliana gene products: Structural and functional analysis of cell and subcellular proteomes". Team coordinator Norbert Rolland. 2000-2001.
• Grant CEA "Action Incitative Chimie". Project "Physiology and genetics of transport of radionuclides in plants". Team coordinator Norbert Rolland. 1999-2001.
• Grant CNRS “Physique et Chimie du Vivant”. Project 1998-122 "Subcellular plant proteome: Subcellular localization of gene products of Arabidopsis thaliana". Project coordinator Norbert Rolland. 1998-2000.